MaayanLab
biojupies-plugins
Python

User-submitted RNA-seq data analysis tools for BioJupies.

Last updated Jul 16, 2025
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README

BioJupies Plugins

Notebooks Generated Datasets Available Plugins

RNA-seq data analysis plugins for the BioJupies project (http://biojupies.cloud).

Source code for the BioJupies web server available at https://github.com/MaayanLab/biojupies.

Overview

What is BioJupies?

BioJupies is a web server which allows users to automatically generate Jupyter Notebooks from RNA-seq datasets through an intuitive interface, with no knowledge of coding required. It can be accessed for free from http://biojupies.cloud.

Screenshot of the BioJupies website landing page.

What are BioJupies plugins?

BioJupies plugins are modular snippets of code which can analyze RNA-seq data and embed visualizations, interactive plots, or analysis results in Jupyter Notebooks.

Plugins may perform a variety of different analyses, ranging from exploratory data analysis visualizations, differential gene expression, clustering, enrichment analysis, and small molecule queries. A full list of the plugins available is displayed below.

This repository contains the source code for all BioJupies plugins, written in Python 3 and R.

What plugins are currently available?

The BioJupies RNA-seq analysis toolbox currently contains the following plugins:

| Plug-in Name | Description | | --- | --- | | Clustergrammer | Interactive hierarchical clustering heatmap visualization | | Library Size Analysis | Analysis of readcount distribution for the samples within the dataset | | PCA | Linear dimensionality reduction technique to visualize similarity between samples | | MA Plot | Plot the logFC and average expression values resulting from a differential expression analysis | | Differential Expression Table | Differential expression analysis between two groups of samples | | Volcano Plot | Plot the logFC and logP values resulting from a differential expression analysis | | Enrichr Links | Links to enrichment analysis results of the differentially expressed genes via Enrichr | | Gene Ontology Enrichment Analysis | Identifies Gene Ontology terms which are enriched in the differentially expressed genes | | Kinase Enrichment Analysis | Identifies protein kinases whose substrates are enriched in the differentially expressed genes | | miRNA Enrichment Analysis | Identifies miRNAs whose targets are enriched in the differentially expressed genes | | Pathway Enrichment Analysis | Identifies biological pathways which are enriched in the differentially expressed genes | | Transcription Factor Enrichment Analysis | Identifies transcription factors whose targets are enriched in the differentially expressed genes | | L1000CDS2 Query | Identifies small molecules which mimic or reverse a given differential gene expression signature | | L1000FWD Query | Projects signatures on a 2-dimensional visualization of the L1000 signature database |

Can I contribute my RNA-seq analysis plugins?

Users are welcome to contribute RNA-seq data analysis plugins for integration in BioJupies.

To contribute your plugin, follow our submission tutorial or visit the contribute page on this GitHub repository.

References

BioJupies: Automated Generation of Interactive Notebooks for RNA-seq Data Analysis in the Cloud Torre, D., Lachmann, A., and Ma’ayan, A. (Cell Systems, 2018)

License

This project is licensed under the Apache-2.0 License - see the LICENSE.md file for details
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